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Defined three-dimensional microenvironments boost induction of pluripotency
Related publications (33)
Deciphering the molecular events leading to the global restoration of the chromatin landscape during mitotic exit
Two fundamental properties of embryonic stem cells (ESCs) are their ability to self-renew and differentiate into all somatic cell types. Maintenance of their identity faces major challenges when transitioning through mitosis, as most DNA-binding proteins a ...
EPFL2023ISSCR standards for the use of human stem cells in basic research
The laboratory culture of human stem cells seeks to capture a cellular state as an in vitro surrogate of a biological system. For the results and outputs from this research to be accurate, meaning-ful, and durable, standards that ensure reproducibility and ...
Cambridge2023Programming of Multicellular Patterning with Mechano-Chemically Microstructured Cell Niches
Multicellular patterning of stem-cell-derived tissue models is commonly achieved via self-organizing activities triggered by exogenous morphogenetic stimuli. However, such tissue models are prone to stochastic behavior, limiting the reproducibility of cell ...
WILEY2023Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix
Philippe Renaud, Thomas Michael Braschler, Fatemeh Navaee
Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employi ...
MDPI2023Generation of Human iPSC-Derived Neurons on Nanowire Arrays Featuring Varying Lengths, Pitches, and Diameters
Anna Fontcuberta i Morral, Wonjong Kim
Nanowire (NW) arrays interfaced with biological cells have been demonstrated to be potent tools for advanced applications such as sensing, stimulation, or drug delivery. Many implementations, however, have so far only been studied with rather robust basic ...
WILEY2022The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells
Precise spatiotemporal regulation of gene expression is essential for development and homeostasis of complex organisms. This is achieved in large part by sequence-specific transcription factors (TF) that bind to genomic regulatory elements to activate or r ...
EPFL2019Quantitative analysis of SOX2 and OCT4 expression in pluripotency and differentiation
Embryonic stem (ES) cells have the capacity to give rise to all cell types of the adult organism and can be used as a model to study early cell fate decisions as they closely recapitulate in vivo events. The M and G1 phases have been suggested to be the ke ...
EPFL2018In vivo modeling of human neuron dynamics and Down syndrome
Harnessing the potential of human stem cells for modeling the physiology and diseases of cortical circuitry requires monitoring cellular dynamics in vivo. We show that human induced pluripotent stem cell (iPSC)-derived cortical neurons transplanted into th ...
AMER ASSOC ADVANCEMENT SCIENCE2018Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions
Amniotic fluid stem cells (AFSC) represent an attractive potential cell source for fetal and pediatric cell-based therapies. However, upgrading them to pluripotency confers refractoriness toward senescence, higher proliferation rate and unlimited different ...
Taylor & Francis Inc2016A role for mitotic bookmarking of SOX2 in pluripotency and differentiation
Suliana Manley, David Michael Suter, Marion Leleu, Cédric Deluz, Aleksandr Benke, Andrea Callegari, Torgil Elias Friman, Mahé Cécile Raccaud, Daniel Strebinger
Mitotic bookmarking transcription factors remain bound to chromosomes during mitosis and were proposed to regulate phenotypic maintenance of stem and progenitor cells at the mitosis-to-G1 (M-G1) transition. However, mitotic bookmarking remains largely unex ...
Cold Spring Harbor Laboratory2016