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Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy

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Fast focus field calculations

Theo Lasser, Marcel Leutenegger, Rainer Leitgeb

We present a method for fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye diffraction integral is evaluated with the fast Fourier transform f ...

2008

k-Microscopy: resolution beyond the diffraction limit

Theo Lasser, Rainer Leitgeb

We introduce a novel microscopy method of probing the spatial frequencies of a two dimensional sample with a single point detector allowing for sub- micron resolution with low NA lenses as well as for lateral phase resolution in the nanometer range. We syn ...

2008

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Different interferometric techniques were developed last decade to obtain full field, quantitative, and absolute phase imaging, such as phase-shifting, Fourier phase microscopy, Hilbert phase microscopy or digital holographic microscopy (DHM). Although, th ...

SPIE2008

Fast focus field calculations

Theo Lasser, Marcel Leutenegger, Rainer Leitgeb

SPIE2008

Recently, we have experimentally demonstrated a new form of cross-sectional, coherence-gated fluorescence imaging referred to as SD-FCT (‘spectral-domain fluorescence coherence tomography’). Imaging in SD-FCT is accomplished by spectrally detecting self-in ...

2007

Shot noise, refractive index tomography and aberrations estimation in digital holographic microscopy

Florian Charrière

The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...

EPFL2007

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Christian Depeursinge, Tristan Colomb, Frédéric Montfort, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Optical coherence tomography - high resolution imaging of structure and function

Rainer Leitgeb

There is no doubt that optical coherence tomography has become an important biomedical imaging tool today especially due to recent improvements in detection as well as fight source technology. OCT achieves resolution in tissue of a few micrometers and can ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2007

This thesis presents a new tomographic imaging method using multiple wavelengths in digital holographic microscopy. It is based on the addition of several reconstructed wavefronts measured at different wavelengths. The resulting diffraction tomographic vis ...

EPFL2006

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We report on a new detection scheme for Fourier domain optical coherence microscopy that exhibits high transverse resolution along an axially extended focal range. Nearly constant transverse resolution of ∼1.5 μm along a focal range of 200 μm is experiment ...

2006