Publications related to Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy

Anthracene-Tethered Ruthenium(II) Arene Complexes as Tools To Visualize the Cellular Localization of Putative Organometallic Anticancer Compounds

Paul Joseph Dyson, Christian Hartinger, Wee Han Ang, Alexey Nazarov, Olivier Pierre Zava, Michael Groessl, Albert Ruggi, Frédéric Schmitt, Julie Risse

Anthracene derivatives of ruthenium(II) arene compounds with 1,3,5-triaza-7-phosphatricyclo[3.3.1.1]decane (pta) or a sugar phosphite ligand, viz., 3,5,6-bicyclophosphite1,2-O-isopropylidene-alpha-D-glucofuranoside, were prepared in order to evaluate their ...

2012

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Jeremy Luterbacher

We report on studies of Thermobifida fusca cellulases Cel5A, Cel6B and Cel9A binding to pretreated wood particles using Confocal Laser Scanning Microscopy (CLSM). Hydro-thermal pretreated wood particles were immobilized on borosilicate substrates before fl ...

2011

Comparison between SOFI and STORM

Theo Lasser, Stefan Geissbühler, Claudio Dellagiacoma

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time usi ...

2011

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag

Kai Johnsson, Sambashiva Banala, Mako Kamiya

We present a method for the specific labelling of fusion proteins with synthetic fluorophores in Zebrafish. The method uses the SNAP-tag technology and O-6-benzylguanine derivatives of various synthetic fluorophores. We demonstrate how the method can be us ...

Wiley-Blackwell2011

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Giovanni Dietler, Mounir Driss Mensi, Serguei Sekatski, Kanat Dukenbayev

A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor?ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. T ...

2010

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Christian Depeursinge, Christophe Pache, Jonas Kühn, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic Microscopy (DHM) and widefield epifluorescence microscopy modes in a time seq ...

SPIE2010

Real Time 3-D Cytomorphological Imaging Using Digital Holographic Microscopy and Fluorescence Microscopy for Space Biology

Christian Depeursinge, Christophe Pache, Jonas Kühn, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli

A microscope operating in Digital Holographic Microscopy (DHM) and classical widefield epi-fluorescence microscopy in a time sequential manner is developed to study morphological alterations of mouse myoblast cells under simulated microgravity in real time ...

OSA2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

High-resolution microscopy and photolithography devices using focusing micromirrors

René Salathé, Fabrice Merenda, Bastien Rachet

The invention relates to large-field high-resolution microscopy and photolithography setups operating with polychromatic light. It includes the use of a plurality of focusing micromirrors. ...

2010

Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy

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Anthracene-Tethered Ruthenium(II) Arene Complexes as Tools To Visualize the Cellular Localization of Putative Organometallic Anticancer Compounds

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Comparison between SOFI and STORM

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Real Time 3-D Cytomorphological Imaging Using Digital Holographic Microscopy and Fluorescence Microscopy for Space Biology

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

High-resolution microscopy and photolithography devices using focusing micromirrors

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Anthracene-Tethered Ruthenium(II) Arene Complexes as Tools To Visualize the Cellular Localization of Putative Organometallic Anticancer Compounds

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Real Time 3-D Cytomorphological Imaging Using Digital Holographic Microscopy and Fluorescence Microscopy for Space Biology

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

High-resolution microscopy and photolithography devices using focusing micromirrors

Comparison between SOFI and STORM