Publications related to Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Christian Depeursinge, Christophe Pache, Jonas Kühn, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic Microscopy (DHM) and widefield epifluorescence microscopy modes in a time seq ...

SPIE2010

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

Superresolution Imaging using Single-Molecule Localization

Suliana Manley

Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (similar to 10-20-nm resolution), allowing biological processes to be described at the ...

2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Real Time 3-D Cytomorphological Imaging Using Digital Holographic Microscopy and Fluorescence Microscopy for Space Biology

Christian Depeursinge, Christophe Pache, Jonas Kühn, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli

A microscope operating in Digital Holographic Microscopy (DHM) and classical widefield epi-fluorescence microscopy in a time sequential manner is developed to study morphological alterations of mouse myoblast cells under simulated microgravity in real time ...

OSA2010

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Marcel Leutenegger

Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular autofluorescence is minimi ...

2010

Comparison of Deconvolution Software: A User Point of View—Part 2

Daniel Sage, Alessandra Griffa

Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...

EMS2010

Microscopy image resolution improvement by deconvolution of complex fields

Christian Depeursinge, Nicolas Pavillon, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

Based on truncated inverse filtering, a theory for deconvolution of complex fields is studied. The validity of the theory is verified by comparing with experimental data from digital holographic microscopy (DHM) using a high-NA system (NA=0.95). Comparison ...

Optical Society of America2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy

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Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Superresolution Imaging using Single-Molecule Localization

Three-dimensional nano-localization of single fluorescent emitters

Real Time 3-D Cytomorphological Imaging Using Digital Holographic Microscopy and Fluorescence Microscopy for Space Biology

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Comparison of Deconvolution Software: A User Point of View—Part 2

Microscopy image resolution improvement by deconvolution of complex fields

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Three-dimensional nano-localization of single fluorescent emitters

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Superresolution Imaging using Single-Molecule Localization

Real Time 3-D Cytomorphological Imaging Using Digital Holographic Microscopy and Fluorescence Microscopy for Space Biology

Comparison of Deconvolution Software: A User Point of View—Part 2

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Microscopy image resolution improvement by deconvolution of complex fields

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity