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Phase Reconstruction of Low-Energy Electron Holograms of Individual Proteins

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Amplitude and phase reconstruction for Low-Energy Electron Holography of individual proteins

Hannah Julia Ochner

Single-molecule imaging methods are of importance in structural biology, and specifically in the imaging of proteins, since they can elucidate conformational variability and structural changes that might be lost in imaging methods relying on averaging proc ...

EPFL2022

Structure determination techniques are an essential tool to investigate and understand molecules, especially in biology, where functionality crucially depends on structure. The dominant high-resolution methods, such as transmission electron microscopy and ...

EPFL2022

Stochasticity helps to navigate rough landscapes: comparing gradient-descent-based algorithms in the phase retrieval problem

Lenka Zdeborová, Francesca Mignacco

In this paper we investigate how gradient-based algorithms such as gradient descent (GD), (multi-pass) stochastic GD, its persistent variant, and the Langevin algorithm navigate non-convex loss-landscapes and which of them is able to reach the best general ...

IOP PUBLISHING LTD2021

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

We report on a 4-bit digital silicon photonic MEMS phase-shifter with sub-microsecond switching time and low power consumption (< 10 uW). The device consists of 4 digitally switched phase-shifting units, which in total enable phase shift from pi/8 to 15pi/ ...

IEEE2018

Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...

EPFL2017

Hermite Snakes with Control of Tangents

Michaël Unser, Julien René Pierre Fageot, Virginie Sophie Uhlmann

We introduce a new model of parametric contours defined in a continuous fashion. Our curve model relies on Hermite spline interpolation and can easily generate curves with sharp discontinuities; it also grants direct access to the tangent at each location. ...

IEEE2016

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Pierre Magistretti, Christian Depeursinge, Benjamin Rappaz, Pierre Marquet, Pascal Jourdain, Kaspar Rothenfusser

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...

Spie-Int Soc Optical Engineering2016

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Microstructures can be used to realize repetitive and singular high contrast features at different distances behind a structure. For practical applications, the amplitude field needs to be considered. To realize defined amplitude features at long distances ...

Spie-Int Soc Optical Engineering2016

High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

Jean-Jacques Meister, Charles Brepsant, Mark Etienne Ambühl

Accurate extraction of cell outlines from microscopy images is essential for analysing the dynamics of migrating cells. Phase-contrast microscopy is one of the most common and convenient imaging modalities for observing cell motility because it does not re ...

2012