Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles
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The need to image objects on increasingly finer scales and spatially localized specific molecules can be met by the combination of infrared, visible and Raman spectroscopy with scanning near-field microscopy, giving rise to a powerful nanospectroscopic too ...
Super-resolution is a set of techniques used to enhance resolution of image. This work focuses on super resolution used in fluorescent microscopy, ex to image structures in the living cell. In this report we present new method for this task, both in one di ...
We introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluo ...
Optical microscopy has become a major non- or minimally invasive tool for investigating living organisms such as cells, tissues or small animals. Being such an essential tool for research and because lacking knowledge and insight in the working principles ...
Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...
Super-resolution fluorescence microscopy is a promising tool with the potential to strengthen our understanding of living processes. Based on the ability to switch fluorophores on and off in an either deterministic or stochastic manner, the fundamental lim ...
This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...
The field of biological imaging has evolved considerably during the past decade as a result of recent (r)evolutions in fluorescence labeling and optical microscopy. Bioimage informatics has been identified as a top priority to cope with the ever-increasing ...
The possibility to visualize and image the arrangement of proteins within the cell at the molecular level has always been an attraction for scientists in biological research. In particular, for signalling molecules such as GPCRs (G-protein-coupled receptor ...
With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...