Concept
In vitro
Publications associées (32)
In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera
Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü
Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM ...
Optica Publishing Group2022Raman microspectroscopy reveals unsaturation heterogeneity at the lipid droplet level and validates an in vitro model of bone marrow adipocyte subtypes
Olaia Maria Naveiras Torres-Quiroga, Charles Christopher Bataclan, Vasco Ledebur de Antas de Campos, Josefine Catharina Pedersen Tratwal, Daniel Naveed Tavakol
Bone marrow adipocytes (BMAds) constitute the most abundant stromal component of adult human bone marrow. Two subtypes of BMAds have been described, the more labile regulated adipocytes (rBMAds) and the more stable constitutive adipocytes (cBMAds), which d ...
FRONTIERS MEDIA SA2022Organoids as tools for fundamental discovery and translation-A Keystone Symposia report
Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to ...
WILEY2022NMR spectroscopy of a single mammalian early stage embryo
Jürgen Brugger, Giovanni Boero, Marco Grisi, Gaurasundar Marc Conley, Kyle Joel Rodriguez
The resolving power, chemical sensitivity and non-invasive nature of NMR have made it an established technique for in vivo studies of large organisms both for research and clinical applications. NMR would clearly be beneficial for analysis of entities at t ...
ACADEMIC PRESS INC ELSEVIER SCIENCE2022Azide-Containing Hypervalent Iodine Reagents for Biomolecules Functionalization
Raphaël Michel Henri Simonet-Davin
The goal of my PhD was the development of a new method for unselective surface labeling, not substrate-specific, that would give access to direct visualization of any surface interactions.In order to achieve this challenging goal, we wanted to develop a ge ...
EPFL2022Novel trehalose-based excipients for stabilizing nebulized anti-SARS-CoV-2 antibody
Sandrine Gerber, François Rémi Pierre Noverraz, Solène Marcelle Françoise Marie Passemard
COVID-19 is caused by the infection of the lungs by SARS-CoV-2. Monoclonal antibodies, such as sotrovimab, showed great efficiency in neutralizing the virus before its internalization by lung epithelial cells. However, parenteral routes are still the prefe ...
2022In-vitro and in-silico characterization of zein fiber incorporating cuminaldehyde
The zein solutions containing different concentrations of cuminaldehyde (5%, 10%, and 20%, w/w) were electrospun. The morphology and average diameter of fibers were evaluated by scanning electron microscopy and the optimized fiber (20% cuminaldehyde) was c ...
ELSEVIER2021Insights gained from a comprehensive all-against-all transcription factor binding motif benchmarking study
Giovanna Ambrosini, Philipp Bucher, Romain Fernand Pietro Groux
BackgroundPositional weight matrix (PWM) is a de facto standard model to describe transcription factor (TF) DNA binding specificities. PWMs inferred from in vivo or in vitro data are stored in many databases and used in a plethora of biological application ...
2020Extending the Concept of Predicting Fish Acute Toxicity In Vitro to the Intestinal Cell Line RTgutGC
Kristin Schirmer, Hannah Inka Schug, Jenny Maner
Testing chemicals for fish acute toxicity is a legal requirement in many countries as part of environmental risk assessment. To reduce the number of fish used, substantial efforts have been focused on alternative approaches. Prominently, the cell viability ...
SPEKTRUM AKADEMISCHER VERLAG-SPRINGER-VERLAG GMBH2020