Harmonique (musique) | EPFL Graph Search

vignette|Les harmoniques d'une corde vibrante En acoustique musicale, un partiel harmonique (ou, plus simplement, un harmonique) est une composante d’un son périodique, dont la fréquence est un multiple entier d'une fréquence fondamentale. Dans une conception traditionnelle du « son musical », le son musical est périodique, c'est-à-dire que ses partiels sont harmoniques ; les sons non harmoniques étaient décrits comme des « bruits ». Dans les musiques d'aujourd'hui, tout son peut être considéré comme musical dans un contexte approprié. La fondamentale d'un son périodique détermine la hauteur perçue, la puissance relative des partiels harmoniques de rangs supérieurs influe, avec des caractères dynamiques, sur le timbre. En musique on appelle « harmoniques » les sons qu'on obtient, sur les instruments à cordes, en forçant la vibration d'une corde à un mode supérieur à son mode fondamental. Par exemple, en effleurant la corde au tiers de sa longueur, on empêche son déplacement latéral

Second harmonic scattering of water in a biological context

Tereza Schönfeldová

Water is one of the most abundant molecules in the universe. It forms a hydrogen bond network with unique structure and dynamics. Hydrogen bonding is highly relevant to many biological processes including membrane formation, protein folding, adsorption, and lubrication. However, the underlying molecular interactions within water and between water and membranes are difficult to study and hence not fully understood. In this thesis, we utilize second harmonic scattering (SHS) to investigate inhomogeneities in bulk water, probe structural changes in lipid bilayer membranes, and detect and characterize protein-membrane interactions. Firstly, we explore the structure of bulk water in comparison to a common model liquid, carbon tetrachloride. Combining SHS measurements and molecular dynamics simulations, we report on the nanoscale inhomogeneities in bulk water that occur on femtosecond timescales. We attribute the rise in the coherent second harmonic (SH) intensity to charge density fluctuations with enhanced nanoscale polarizabilities around transient voids. Even though the transient voids also exist in carbon tetrachloride, they do not show polarizability fluctuations and the SH response of carbon tetrachloride thus corresponds to that of a model isotropic liquid. Then, we study the structure of water around large unilamellar vesicles (LUVs), which serve as a model for cell membranes. We focus on the role of water during the lipid melting phase transition. Using SHS, differential scanning calorimetry, and temperature-dependent zeta-potential measurements, we investigate the structural changes of hydration shells around the vesicle membrane. We use lipids with phosphoserine, phosphocholine, and phosphate headgroups and observe different hydration behavior upon the lipid melting transition depending on the membrane composition. Next, we characterize the interface of membranes composed of lipids containing phosphocholine and phosphate headgroups in solutions with different CaCl2 concentrations. We observe that the SH intensity is decreasing with the addition of CaCl2, which we attribute to the formation of a condensed layer of Na+ counterions on the inner leaflet of the LUVs. Additionally, we extract the vesicle surface potential and second-order surface susceptibility from SHS data for membranes with different content of lipids with phosphate headgroups, and observe composition dependent behaviour which suggests structural membrane reorganization. We continue by exploring protein-membrane interactions using water as a contrast agent. We study a pore-forming toxin, perfringolysin O (PFO), known for selective insertion into cholesterol-rich membranes. Combining SHS measurements and cryo-electron microscopy, we compare the interaction between PFO and LUV membranes with a high and low cholesterol content. By obtaining a sub-picomolar insertion constant of PFO, we show that SHS can be used to determine protein-membrane binding constants in concentration ranges not accessible by conventional methods.Finally, we investigate the interaction of protein aerolysin and its mutants with lipid membranes. We observe changes in surface charge in the cap region of membrane-bound aerolysin at different pH of the surrounding solution. By combining SHS with single-channel measurements, we obtain the membrane binding constant of wild-type aerolysin and its mutants, and we dissect their pore-forming mechanism.

EPFL2022

Binaural localization and separation techniques

Harald Viste

Abstract Based on binaural signals, i.e. the signals observed at the two ears, a listener can localize and recognize different sound sources and then focus on one of these. For decades, researchers have tried to invent a machine that can do the same under similar conditions. Despite all the efforts, the human auditory system is, by far, superior to any machine that has been devised. The topic of this thesis is computational techniques for the localization and separation of sources in binaural signals. In order to give an overview of different areas of research that have considered the problems of source localization and separation, we start with a review of existing techniques. This provides the background for the techniques that we propose subsequently. Binaural Localization The most important cues for localization of sound sources in binaural signals are the level and time differences between the ears. We propose a technique for the joint evaluation of these cues where noisy level difference estimates are combined with less noisy but ambiguous time difference estimates in order to provide accurate azimuth estimates. The proposed technique enables the localization of sources and the tracking of these in dynamic scenes. Head model Based on a study of the level and time differences as function of azimuth angle for different heads, we propose a generic model that is parametrized by the distance between the ears only. This enables the use of the binaural localization technique mentioned above for a listener whose head related transfer functions have not been measured. Binaural separation For the separation of sources we propose a method based on spatial windowing in the azimuth parameter space. Separation of overlapping partials Finally, we propose a technique for the separation of overlapping partials in mixtures of harmonic instruments. The technique is based on the similarity of temporal envelopes between the different partials of a harmonic note.

EPFL2004

Device and method for measuring and imaging second harmonic and multi-photon generation scattered radiation

Carlos Alberto Macias Romero, Sylvie Roke

Embodiments of the subject invention relate to a method and apparatus for performing measurements using multiphoton or second harmonic generation (SHG) scattered radiation from a sample including a turbid (scattering) medium includes providing a beam of laser pulses from a laser source having high pulse energies and a repetition rate; splitting the beam of laser pulses into two or more partial beams and focussing and overlaying the partial beams on a sample including the turbid medium; and detecting multiphoton and second harmonic radiation scattered from the sample.

2015