Bioremédiation | EPFL Graph Search

La bioremédiation consiste en la décontamination de milieux pollués au moyen de techniques issues de la dégradation chimique ou d'autres activités d'organismes vivants. Introduction Empiler et humidifier des résidus organiques permet la décomposition biologique : un processus connu comme le compostage de déchets organiques. La technologie a été étendue pour inclure le traitement de déchets alimentaires, des déchets agricoles et des eaux usées. Plus récemment, la bioremédiation a été appliquée au traitement des déchets dangereux. C'est-à-dire permettant la remédiation des sols pollués et des eaux usées. Techniques de bioremédiation Atténuation naturelle contrôlée Principe Suivi passif de la diminution des teneurs en polluants par le biais des processus naturels :

Dégradation par la microfaune
Dilution
Absorption
Évaporation

Méthode Il s'agit de laisser les micro-organismes du sol décontaminer naturellement le milieu. Le

Dégradation par la microfaune
Dilution
Absorption
Évaporation

Méthode Il s'agit de laisser les micro-organismes du sol décontaminer naturellement le milieu. Le

Assessment of microbial community changes and limiting factors during bioremediation of hydrocarbon-polluted soil with new miniaturized physiological methods

Karin Kaufmann

Due to human activities, organic pollutants are spilled to the environment where they threaten public health, often as contaminants of soil or groundwater. Living organisms are able to transform or mineralize many organic pollutants, and bioremediation techniques have been developed to remove pollutants from a contaminated site. However, fast and easy methods to document both the efficacy of bioremediation and the changes in soil microbial communities during bioremediation are not well developed. The major aim of this thesis was to develop miniaturized methods targeting the physiology of microbial communities during pollutant degradation in soil and to assess pollution-induced community changes. Furthermore, the methods should identify factors limiting efficient pollutant biodegradation. Petroleum hydrocarbons have been chosen as pollutants because these compounds are frequently spilled, readily water-soluble, partly toxic and carcinogenic, and therefore undesirable in soils and drinking water supplies. The influence of petroleum hydrocarbons on a microbial community in the vadose zone was assessed under field conditions. An artificial hydrocarbon mixture consisting of volatile and semi-volatile compounds similar to jet-fuel was emplaced in a previously uncontaminated vadose zone in nutrient-poor glacial melt water sand. The experiment included monitoring of microbial parameters and CO2 concentrations in soil gas over 3 months, in- and outside the hydrocarbon vapor plume that formed around the buried petroleum. Microbial and chemical analyses of vadose zone samples were performed on material from 9 cores drilled on 3 days at 3 distances from the buried petroleum mass to 3.3 m depth. Significantly elevated CO2 concentrations were observed after contamination. Total cell numbers as determined by fluorescence microscopy were strongly correlated with soil organic carbon and nitrogen content but varied little with contamination. Redundancy analysis (RDA) allowed direct analysis of effects of selected environmental variables or the artificial contamination on microbiological parameters. Variation in biomass and CO2 production was to 46 % explained by soil parameters and to 39.8 % by the duration of contamination. The microbial community structure was assessed by community-level physiological profile analysis using BiologTM EcoPlates. Only 35.9 % of variation in BiologTM data could be attributed to soil parameters and contamination, however, the samples with greatest exposure to hydrocarbons grouped together on RDA plots. It is concluded that at this nutrient-poor site, the microbial community was dominated by the natural heterogeneity and that the influence of petroleum hydrocarbon vapor was weak. BiologTM Eco-Plates combined with RDA were able to distinguish between samples with high and low hydrocarbon exposure. However, the method was not sensitive enough to produce consistent patterns in three replicates when extracts from subsurface soils with low cell numbers were inoculated. A more sensitive, MPN-based system was therefore developed and its ability to provide information about specific pollutant degraders was tested in alluvial sand. Eight dilutions of sand extracts were inoculated on medium- and substrate-containing polypropylene deep-well plates. To keep the concentrations of the volatile substrates constant over several days of incubation without intoxicating the soil community, a substrate-containing organic carrier phase was applied to each vial. The biochemically inert 2,2,4,4,6,8,8-heptamethylnonane (HMN) was appropriate for this purpose. Thus, the volatile substrates partition from HMN into the microorganism-containing soil water where they achieve relatively low concentrations. The sensitive watersoluble tetrazolium dye (WST-1) was added to each well after 7 days incubation to detect dehydrogenase activity. A dark yellow signal was developed for the substrates toluene, n-octane, n-dodecane, 1,2,4-trimethylbenzene and methylcyclohexane but not for isooctane, being strongest in n-alkane amended wells. With this method, more hydrocarbon degrading bacteria were detected in an alluvial sand exposed to kerosene for 72 hours compared to the community in the pristine sand. Both communities were mainly composed of n-alkane degraders. Although encouraging results were achieved, we noticed that the tetrazolium reduction was inhibited in some cases. Wells with high cell numbers, in which obvious growth occurred, did not produce a signal. In a next step, the two-liquid phase system with HMN was applied to modify a miniaturized respiration detection system using whole soil samples instead of soil extracts (Campbell et al., AEM 69, p. 3593). Soil was incubated in deep-well plates in presence of single volatile organic hydrocarbons. The carrier phase was added to the interstitial space in a bead layer on the bottom of the well (below the soil sample), and the hydrocarbons diffused into the soil-water. The soil activity was determined by means of an agar plate containing a pH indicator, which changes color as a function of the produced CO2. Physiological profiles specific to petroleum hydrocarbons in pristine and contaminated soils were assessed. The substrate concentrations, which induced highest microbial activity, as well as concentrations causing inhibition, were determined with effect-concentration curves. Community-level physiological profiles based on hydrocarbon degradation were obtained by applying multivariate analysis on the CO2 yields. The first measurement period of 0-6 hours after addition of relatively high hydrocarbon concentrations to the system yields values, which best separated soil types as well as pristine and contaminated soils. If low concentrations were applied, we obtained only significant separations after 24 hours incubation, probably after a growth step. The same micro-respiration system equipped with a 14CO2 detection plate was applied to study N and P limitation of aerobic mineralization of 14C-toluene in a soil previously exposed to petroleum. Significant nutrient limitation during short-term tests (24-48 hours) was identified and the optimized combination of nutrient addition was determined. Conclusively, miniaturized physiological methods have been proven to detect soil microbial community changes in petroleum hydrocarbon-contaminated soils. Adding an organic carrier phase to the miniaturized systems allowed assuring a volatile organic hydrocarbon supply in non-toxic concentrations in the soil water or the medium for several days. Thus simple, cost-effective systems to investigate the soil community during bioremediation and to address questions of nutrient shortage in soils have been provided with this thesis.

EPFL2004

Reduction and immobilization of uranium in the subsurface

Malgorzata Alicja Stylo

Decades of uranium (U) mining, milling and military use left a legacy of U contamination around the world. The radioactivity and chemical toxicity of U at contaminated sites pose an acute and long-term hazard to human health and the surrounding environment. In order to diminish the risk, in situ bioremediation methods, which contribute to contaminant immobilization, are proposed. Nevertheless, the reported prevalent formation of labile and non-crystalline U(IV) species as a result of microbial U(VI) reduction, in contrast to more stable and crystalline uraninite, undermines the effectiveness of the applied bioremediation. Therefore, a holistic understanding of the controls and mechanisms that govern the formation of non-crystalline U(IV) in the environment is at the core of this thesis. Presence of common groundwater solutes (sulfate, silicate and phosphate) were shown to induce the production of bacterial extracellular polymeric substances (biofilm matrix components), which in turn increases the formation of non-crystalline U(IV) as a result of microbial U reduction. In contrast, a field study suggested that non-crystalline U(IV) was a product of abiotic U reduction followed by the sequestration of U(IV) ions by the biofilm matrix. Those contrasting theories, motivated us to look for an indicator capable of differentiating between biotic and abiotic U reduction in the environment. Uranium isotope fractionation proved to be an excellent tool. Based on our results, the isotopic signature of biotic U reduction (accumulation of 238U in the reduced phase) is easily distinguishable from the abiotic U reduction signature (either no isotopic fractionation or fractionation in the opposite direction). When contrasted with U isotope signatures recorded in the sediments, the findings of this study indicated that biological activity contributed to the formation of many ancient and modern U(IV) deposits. Equipped with a tool capable of assessing the origin of the U(IV) product, and with an understanding of the relationship between the biofilm matrix and the formation of non-crystalline U(IV), we embarked on a project to validate the mechanism responsible for non-crystalline U(IV) formation in the subsurface. Results show that regardless whether U is reduced enzymatically or though abiotic agents, the formed U(IV) species are always non-crystalline if the biofilm matrix is present. However, according to our experiments, biological U reduction resulted in better immobilization and overall reduction of U. The mechanism of U reduction in our systems was shown to be controlled by U speciation, which in turn, is govern by the presence of bicarbonate. Under low carbonate concentrations, the majority of U(VI) adsorbs onto the mineral phase thus promoting abiotic U reduction mediated by redox active minerals. However, at higher carbonate concentrations, most of U(VI) is present as aqueous U(VI)-carbonate complexes precluding sorption and thus allowing for enhanced enzymatic U reduction. The results obtained here shed light on the processes occurring at U-contaminated sites and have several field implications. The effectiveness of applied bioremediation was shown to depend on the geochemical conditions at the field site. However, the injection of bicarbonate during in situ clean-up operation enhances microbial U reduction and by this, the overall reductive immobilization of U.

EPFL2015

In Situ Biostimulation of Cr(VI) Reduction in a Fast-Flowing Oxic Aquifer

Daniel Scott Alessi, Rizlan Bernier-Latmani

Hexavalent chromium is among the most common and hazardous inorganic contaminants in soils and groundwater. A promising in situ bioremediation approach is the delivery of electron donors to stimulate microbially mediated Cr(VI) reduction, producing relatively insoluble Cr(III) precipitates. Even though this strategy has been implemented successfully in the past, it was primarily under favorable hydrological conditions, such as relatively slow groundwater flow velocities. To evaluate whether microbially mediated Cr(VI) reduction can be sustained at high groundwater flow velocities and under nearly oxygen-saturated conditions, we have conducted laboratory-scale column experiments and a field-scale pilot study using molasses as the electron donor. Despite the unfavorable conditions, both experiments provided clear evidence for microbially mediated Cr(VI) reduction. In particular, the well used for injection in the field experiment became anoxic after two months of injection and the Cr(VI) concentration decreased by a factor of six. After stopping the injection, these conditions prevailed for at least four months. In the column experiments, Cr(VI) reduction was accompanied by distinct Cr isotopic fractionation characterized by an enrichment factor of -1.25 parts per thousand. In contrast, Cr isotope data collected from the field experiment were ambiguous, which was because of the complexation of Cr(III) with organic carbon and the heterogeneous distribution of molasses due to the presence of narrow preferential flow paths. A substantial injectivity decrease likely caused by the formation of biofilm and the precipitation of Fe sulfides formed an additional challenge during the field experiment. Our data, however, also suggest that most of the identified challenges could be addressed by limiting the targeted dissolved organic carbon concentrations to about 10 mg/L. In conclusion, the injection of molasses constitutes a promising bioremediation strategy for the long-term treatment of Cr(VI) contamination even at high flow rates and under oxic conditions.

AMER CHEMICAL SOC2020