Publications de Jacques Dubochet

Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles

Davide Demurtas, John Maddocks, Andrzej Stasiak, Jacques Dubochet, Arnaud Amzallag

We use cryo-electron microscopy (cryo-EM) to study the 3D shapes of 94-bp-long DNA minicircles and address the question of whether cyclization of such short DNA molecules necessitates the formation of sharp, localized kinks in DNA or whether the necessary ...

Oxford University Press2009

Aggregation Behavior of Poly(ethylene glycol-bl-propylene sulfide) Di- and Triblock Copolymers in Aqueous Solution

Jeffrey Alan Hubbell, Conlin O'Neil, Jacques Dubochet

Block copolymers of poly(ethylene glycol)-bl-poly(propylene sulfide) (PEG-PPS) have recently emerged as a new macromolecular amphiphile capable of forming a wide range of morphologies when dispersed in water. To understand better the relationship between s ...

2009

Multifunctional Lipid/Quantum-Dots Hybrid Nanocontainers for Controlled Targeting of Live Cells

Horst Vogel, Davide Demurtas, Pierre-Yves Bolinger, Christophe Danelon, Jacques Dubochet, Paulina Izewska, Gopakumar Gopalakrishnan

In this communication, we report on an interesting observation of wide-ranging potential for cellular imaging and manipulation: Hydrophobic QDs can be easily incorporated into the bilayer membrane of lipid vesicles. Such lipid/QD nanocontainer hybrid vesic ...

2006

Are the light-harvesting I complexes from Rhodospirillum rubrum arranged around the reaction center in a square geometry?

Horst Vogel, Henning Paul-Julius Stahlberg, Jacques Dubochet

The basic photosynthetic unit contg. the reaction center and the light-harvesting I complex (RC-LHI) of the purple non-sulfur bacterium Rhodospirillum rubrum was purified and reconstituted into two-dimensional (2D) membrane crystals. Transmission electron ...

1998

The reaction center of the photounit of Rhodospirillum rubrum is anchored to the light-harvesting complex with four-fold rotational disorder

Horst Vogel, Henning Paul-Julius Stahlberg, Jacques Dubochet

The minimal photounit of the photosynthetic membranes of the purple non-sulfur bacterium Rhodospirillum rubrum, comprising the reaction center and the light-harvesting complex has been purified and crystd. in two dimensions in the presence of added phospho ...

1998

Sulfur-bearing lipids for the covalent attachment of supported lipid bilayers to gold surfaces: a detailed characterization and analysis

Shaik Mohammed Zakeeruddin, Horst Vogel, Henning Paul-Julius Stahlberg, Jacques Dubochet, Martha Liley, Claus Duschl

In a recent paper, a new class of synthetic lipids, which self-assemble to form monolayers on gold surfaces, was introduced. The addn. of a monolayer of phospholipids to these layers results in robust supported lipid bilayers designed to reconstitute trans ...

1996

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