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We study (connected) reductive subgroups G of a reductive algebraic group H, where G contains a regular unipotent element of H. The main result states that G cannot lie in a proper parabolic subgroup of H. This result is new even in the classical case H = SL(n, F), the special linear group over an algebraically closed field, where a regular unipotent element is one whose Jordan normal form consists of a single block. In previous work, Saxl and Seitz (1997) determined the maximal closed positive-dimensional (not necessarily connected) subgroups of simple algebraic groups containing regular unipotent elements. Combining their work with our main result, we classify all reductive subgroups of a simple algebraic group H which contain a regular unipotent element.