A process for the in-situ extraction of fermentation broths
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The inhibitory impacts of the bioconversion of L-phenylalanine (L-Phe) to 2-phenylethanol (PEA), a very important natural aroma compd., on the metab. of Saccharomyces cerevisiae Giv 2009 were investigated in batch and chemostat cultures. The bioconversion ...
An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker's yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents we ...
Biosynthesis of glycosylphosphatidylinositol (GPI)-anchored proteins involves the action of a GPI trans-amidase, which replaces the C-terminal GPI signal sequence (GPI-SS) of the primary translation product with a preformed GPI lipid. The transamidation de ...
A new concept for the construction of macrocyclodepsipeptides from simple and readily available starting materials has been developed. The sequence consists of (a) a three-component reaction of an aldehyde, an amino alc., and a dipeptide isocyanate and (b) ...
A new method for the covalent and specific labeling of fusion proteins of carrier proteins (CPs) with small organic molecules has been developed in this work. This technology combines the convenience of expressing genetically tagged reporter proteins with ...
This review sums up the activity in the field of in situ product removal in whole cell bioprocesses over the last 20 yr. It gives a complete summary of ISPR operations with microbial cells and cites a series of interesting ISPR applications in plant and an ...
The Smg proteins Smg5, Smg6 and Smg7 are involved in nonsense-mediated RNA decay (NMD) in metazoans, but no orthologs have been found in the budding yeast Saccharomyces cerevisiae. Sequence alignments reveal that yeast Ebs1p is similar in structure to the ...
At high growth rates, the biomass yield of baker's yeast (Saccharomyces cerevisiae) decreases due to the prodn. of ethanol. For this reason, it is std. industrial practice to use a fed-batch process whereby the specific growth rate, m, is fixed at a level ...
In order to extend the shelf life of refrigerated dough, a new method based on thermo-gelling immobilization is proposed. This slows fermn. at low temps. (
The bioconversion of L-phenylalanine (L-Phe) to 2-phenylethanol (PEA) by the yeast Saccharomyces cerevisiae is limited by the toxicity of the product. PEA extn. by a sep. org. phase in the fermenter is the ideal in situ product recovery (ISPR) technique to ...