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The electrochemical quantification of analytes in droplets of PBS separated by a fluorinated phase was investigated. PDMS-fused silica chips with pyrolysed photoresist electrodes were prepared using a simple fabrication technique and used to analyze droplets in flow. Potentiostatic chronoamperometry provided current readouts consistent with mass transport and the concentration inside the droplets. This paper highlights measurements of dopamine in droplets in T-junction microfluidic chips at unprecedently low concentrations, with a limit of detection of 207 nM and a linear range of 0.21-20 mu M, giving results similar to continuous flow electrochemistry and allowing the analysis in the striatal extracellular range (
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Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
Vasiliki Tileli, Robin Pierre Alain Girod, Michele Bozzetti, Yen-Chun Chen