Protein Phosphorylation: Mechanisms & Regulation

This lecture covers post-translational modifications of proteins, focusing on phosphorylation. It explains the complexity of the proteome due to modifications like acetylation, methylation, and ubiquitination. The lecture details the chemical modifications of amino acids, the most common post-translational modifications, and the structural effects of phosphorylation in proteins. It also delves into the biological functions of post-translational modifications, the specificity of kinases, and the regulation of kinase activity through phosphorylation. The lecture concludes with a discussion on kinase specificity assays and the importance of understanding kinase structure and activation mechanisms.