Lecture

Capillary Electrophoresis: Principles and Applications

Description

This lecture covers the principles and applications of capillary electrophoresis, a technique for separating ionic or ionizable species based on their migration in an electric field. It explains the differences between electrophoresis and chromatography, the migration theory, electroosmosis phenomenon, and the factors influencing migration rates. The lecture also discusses the use of buffers, capillary modifications, injection methods, detectors, and the resolution of peaks. Additionally, it explores the impact of applied power on separation efficiency, the role of electroosmotic flow, and the potential of electrophoresis on microchips. Various aspects such as chemical derivatization, selectivity, and the use of stationary phases in electrochromatography are also addressed.

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