Real-time polymerase chain reactionA real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively and semi-quantitatively (i.e., above/below a certain amount of DNA molecules).
Reverse transcription polymerase chain reactionReverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR).
Public copyright licenseA public license or public copyright licenses is a license by which a copyright holder as licensor can grant additional copyright permissions to any and all persons in the general public as licensees. By applying a public license to a work, provided that the licensees obey the terms and conditions of the license, copyright holders give permission for others to copy or change their work in ways that would otherwise infringe copyright law. Some public licenses, such as the GNU GPL and the CC BY-SA, are also considered free or open copyright licenses.
Function pointerA function pointer, also called a subroutine pointer or procedure pointer, is a pointer referencing executable code, rather than data. Dereferencing the function pointer yields the referenced function, which can be invoked and passed arguments just as in a normal function call. Such an invocation is also known as an "indirect" call, because the function is being invoked indirectly through a variable instead of directly through a fixed identifier or address. Function pointers allow different code to be executed at runtime.
Public-domain-equivalent licensePublic-domain-equivalent license are licenses that grant public-domain-like rights and/or act as waivers. They are used to make copyrighted works usable by anyone without conditions, while avoiding the complexities of attribution or license compatibility that occur with other licenses. No permission or license is required for a work truly in the public domain, such as one with an expired copyright; such a work may be copied at will.
Polymerase chain reactionThe polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993.
Open accessOpen access (OA) is a set of principles and a range of practices through which research outputs are distributed online, free of access charges or other barriers. Under some models of open access publishing, barriers to copying or reuse are also reduced or removed by applying an open license for copyright. The main focus of the open access movement is "peer reviewed research literature". Historically, this has centered mainly on print-based academic journals.
Anonymous functionIn computer programming, an anonymous function (function literal, lambda abstraction, lambda function, lambda expression or block) is a function definition that is not bound to an identifier. Anonymous functions are often arguments being passed to higher-order functions or used for constructing the result of a higher-order function that needs to return a function. If the function is only used once, or a limited number of times, an anonymous function may be syntactically lighter than using a named function.
Free licenseA free license or open license is a license which allows others to reuse another creator’s work as they wish. Without a special license, these uses are normally prohibited by copyright, patent or commercial license. Most free licenses are worldwide, royalty-free, non-exclusive, and perpetual (see copyright durations). Free licenses are often the basis of crowdsourcing and crowdfunding projects.
In vitroIn vitro (meaning in glass, or in the glass) studies are performed with microorganisms, cells, or biological molecules outside their normal biological context. Colloquially called "test-tube experiments", these studies in biology and its subdisciplines are traditionally done in labware such as test tubes, flasks, Petri dishes, and microtiter plates.
