Concept

Nucleic acid methods

Publications associées (12)

Phosphatidylinositol-4-phosphate: The Golgi and beyond

Initially identified as a key phosphoinositide that controls membrane trafficking at the Golgi complex, phosphatidylinositol-4-phosphate (PI4P) has emerged as a key molecule in the regulation of a diverse array of cellular functions. In this review we will ...

2013

A Portable Setup for Molecular Detection by Transmission LSPR

Carlotta Guiducci, Luca Benini, Enrico Accastelli, Giulia Cappi, Fabio Mario Spiga

In the framework of bioanalytics and multiple array detection, we developed a fully portable and low-cost detection system based on Localized Surface Plasmon Resonance (LSPR) in a transmission configuration (T-LSPR). The transmission approach is suitable t ...

2012

Protein-Binding Microarray Analysis of Tumor Suppressor AP2 alpha Target Gene Specificity

Horst Vogel, Luigino Grasso, Jan Kerschgens, Tanja Egener-Kuhn, Nicolas Mermod, Hans-Anton Lehr

Cheap and massively parallel methods to assess the DNA-binding specificity of transcription factors are actively sought, given their prominent regulatory role in cellular processes and diseases. Here we evaluated the use of protein-binding microarrays (PBM ...

Public Library of Science2011

Bioconjugated lanthanide luminescent helicates as multilabels for lab-on-a-chip detection of cancer biomarkers

Martinus Gijs, Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Venkataragavalu Sivagnanam, Bo Song, Vanesa Fernandez Moreira, Hans-Anton Lehr

The lanthanide binuclear helicate [Eu2(LC2(CO2H))3] is coupled to avidin to yield a luminescent bioconjugate EuB1 (Q ¼ 9.3%, s(5D0) ¼ 2.17 ms). MALDI/TOF mass spectrometry confirms the covalent binding of the Eu chelate and UV-visible spectroscopy allows o ...

2010

The importance of the photonic mode density in bioassays based on evanescent optical waves

Akihiro Sato

We have determined limits of detection (LODs) of DNA hybridization using an optical detection by two different method, i.e., surface plasmon fluorescence spectroscopy (SPFS) and optical waveguide fluorescence spectroscopy (OWFS), however, an identical sens ...

2009

Methods of nucleic acid amplification and sequencing

Gerardo Turcatti

Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified o ...

2008

Electroacoustic Polymer Microchip as an Alternative to Quartz Crystal Microbalance for Biosensor Development

Hubert Girault

Laser photoablation of poly(ethylene terephthalate) (PET), a flexible dielectric organic polymer, was used to design an acoustic miniaturized DNA biosensor. The microchip device includes a 100-μm-thick PET layer, with two microband electrodes patterned in ...

2008

Manipulation of magnetic microparticles in liquid phases for on-chip biomedical analysis methods

Ulrike Lehmann

Magnetic microparticles and their application in bioanalytical microfluidic systems have been steadily gaining interest in recent years. This progress is fueled by the comparatively large and long range magnetic forces that can be obtained independently of ...

EPFL2008

Two-Dimensional Magnetic Droplet Manipulation Platform for Miniaturized Bioanalytical Applications

Martinus Gijs, Ulrike Lehmann, Virendra Kumar Parashar, Caroline Vandevyver

We present a new approach for the manipulation of small liquid samples for the miniaturization of bioanalytical systems. In our system, minute liquid volumes are manipulated via the interaction of a changeable magnetic field and magnetic microparticles con ...

2007

Chemical approaches to microarrays and multifunctional protein labeling

India Christina Sielaff

With the human genome and many other eukaryotic and procaryotic genomes sequenced, the interest in life sciences is shifting towards the examination of the function, modification and regulation of the encoded proteins. Proteins are involved in virtually al ...

EPFL2006

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