A moss bioreactor is a photobioreactor used for the cultivation and propagation of mosses. It is usually used in molecular farming for the production of recombinant protein using transgenic moss. In environmental science moss bioreactors are used to multiply peat mosses e.g. by the Mossclone consortium to monitor air pollution. Moss is a very frugal photoautotrophic organism that has been kept in vitro for research purposes since the beginning of the 20th century. The first moss bioreactors for the model organism Physcomitrella patens were developed in the 1990s to comply with the safety standards regarding the handling of genetically modified organisms and to gain sufficient biomass for experimental purposes. The moss bioreactor is used to cultivate moss in a suspension culture in agitated, and aerated liquid medium. The culture is kept under lighting with temperature and pH value held constant. The culture medium—often a minimal medium—contains all nutrients and minerals needed for growth of the moss. To ensure a maximum growth rate, the moss is kept at the protonema stage by continuous mechanical disruption, e.g. by using rotating blades. Once the density of the culture has reached a certain threshold, the lack of nutrients and the increasing concentration of phytohormones in the medium triggers the differentiation of the protonema to the adult gametophyte. At this point the culture has to be diluted with fresh medium if it is intended for further use. According to the intended yield, this basic principle can be adapted to various types and sizes of bioreactors. The cultivation chamber can, for example, consist of a column, a tube, or exchangeable plastic bags. Various biopharmaceuticals have already been produced using moss bioreactors. Ideally, the recombinant protein can be directly purified from the culture medium. One example for this production method is factor H: this molecule is part of the human complement system. Defects in the corresponding gene are associated with human diseases such as severe kidney and retinal disorders.

À propos de ce résultat
Cette page est générée automatiquement et peut contenir des informations qui ne sont pas correctes, complètes, à jour ou pertinentes par rapport à votre recherche. Il en va de même pour toutes les autres pages de ce site. Veillez à vérifier les informations auprès des sources officielles de l'EPFL.
Cours associés (5)
ChE-311: Biochemical engineering
This course introduces the basic principles of bioprocess engineering and highlights the similarities and differences with chemical engineering. Without going into the fundamentals, it proposes an ove
ChE-320: Bioreactor modeling and simulation
The course of Bioreactor modeling and simulation focuses on the principles of algorithmic design and analysis of biochemical reactors. The application of these designed reactors would be in the produc
ChE-437: Bioprocesses and downstream processing
This course aims at a more advanced coverage of the basic aspects discussed in module ChE-311. It is however of a stand-alone nature, and even students who have little knowledge on - but a keen intere
Afficher plus
Séances de cours associées (8)
Bioréacteurs : cinétique et modélisation
Couvre la cinétique de croissance, les coefficients de rendement et les bioréacteurs alimentés par lots en génie des bioprocédés.
Bioréacteurs : performance et design
Couvre la définition et la performance des bioréacteurs, les matériaux utilisés, les critères de sélection et les types de bioréacteurs.
Bioréacteurs Fed-Batch
Explore le fonctionnement et la conception des bioréacteurs alimentés par lots pour améliorer la productivité de la fermentation et surmonter l'inhibition du substrat.
Afficher plus
Publications associées (17)

Co-culture of microalgae-activated sludge for wastewater treatment and biomass production: Exploring their role under different inoculation ratios

Florian Frédéric Vincent Breider

In this study, mixed culture (microalgae:activated sludge) of a photobioreactor (PBR) were investigated at different inoculation ratios (1:0, 9:1, 3:1, 1:1, 0:1 wt/wt). This work was not only to determine the optimal ratio for pollutant remediation and bio ...
2020

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Marc Moniatte, Dominique Terry Monteil Jr, Valentin Juvet

Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer ra ...
American Chemical Society2016

Constant-yield control of continuous bioreactors

Georgios Savoglidis

The process of anaerobic digestion is a common application in the treatment of industrial and communal wastewater treatment plants. The biochemical reactions taking place inside the fermenter can be described by a system of first order differential equatio ...
Elsevier Science Sa2013
Afficher plus
Concepts associés (1)
Bioréacteur
vignette|Principe du bioréacteur Un bioréacteur, appelé également fermenteur ou propagateur, est un appareil dans lequel on multiplie des micro-organismes (levures, bactéries, champignons microscopiques, algues, cellules animales et végétales) pour la production de biomasse (écologie), ou pour la production d'un métabolite ou encore la bioconversion d'une molécule d'intérêt. Dans les années 1800, Pasteur, Kutzing, Schwann, et Cagniard-Latour ont démontré que la fermentation était causée par des levures, qui sont des organismes vivants (Hochfeld, 2006).

Graph Chatbot

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.