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Concept
Cell counting
Résumé
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry, with applications in research and clinical practice. For example, the complete blood count can help a physician to determine why a patient feels unwell and what to do to help. Cell counts within liquid media (such as blood, plasma, lymph, or laboratory rinsate) are usually expressed as a number of cells per unit of volume, thus expressing a concentration (for example, 5,000 cells per milliliter).
Numerous procedures in biology and medicine require the counting of cells. By the counting of cells in a known small volume, the concentration can be mediated. Examples of the need for cell counting include:
In medicine, the concentration of various blood cells, such as red blood cells and white blood cells, can give crucial information regarding the health situation of a person (see: complete blood count).
In cell therapy, to control the dose of cells administered to a patient.
Similarly, the concentration of bacteria, viruses and other pathogens in the blood or in other bodily fluids can reveal information about the progress of an infectious disease and about the degree of success with which the immune system is dealing with the infection.
The cell concentration needs to be known for many experiments in molecular biology, in order to adjust accordingly the amount of reagents and chemicals that are to be applied in the experiment.
Studies that examine the growth rate of microorganisms (in other words, how fast they divide to create new cells) require cell counting.
Calculating the fraction of dead to live cells as a measure of cell viability, such as of cells exposed to poison.
There are several methods for cell counting. Some are primitive and do not require special equipment, thus can be done in any biological laboratory, whereas others rely on sophisticated electronic appliances.
Source officielle
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