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Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...
Microscopy is of high interest for biology since it allows imaging features that are too small to
be seen with naked eyes. However, cells are mostly transparent to visible and infrared light
which makes it difficult to see with a traditional microscope. To ...
Far field pattern generation is investigated for thick sinusoidal phase grating under a Gaussian beam illumination to obtain a high field of view. We compare the Fraunhofer approximation with the High-NA propagator and demonstrate that the Fraunhofer appro ...