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This lecture covers the process of 3D volume reconstruction using FIB/SEM microscopy, focusing on particle recognition, edge detection, and watershed separation techniques. It also discusses the quantitative microstructure analysis, algorithms for object recognition, and stereological correction. The instructor explains the importance of voxel size, cube dimensions, and area of image data used for 3D reconstruction. Additionally, the lecture delves into the spatial resolution of BSE electrons, scatter range in Nb Sn, and the use of FIB nanotomography for volume reconstruction. Various applications such as analyzing superconducting cables, solid oxide fuel cells, and biocompatible materials are explored.
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