This lecture covers the operation of non-contact AFM, including the avoidance of tip sticking, resonance of the cantilever, feedback mechanisms, force regimes, and types of AFM cantilevers. It also discusses the construction of AFM, image processing techniques, tip effects on images, calibration standards, and advanced SPM modes. Additionally, it explores the concept of faster AFM, lateral force AFM, and force curves in both contact and intermittent contact modes.