Explores liquid chromatography principles, various chromatographic techniques, HPLC components, and the comparison between off-gel IEF and SCX chromatography.
Covers the basics of proteomics, mass spectrometry, protein synthesis, and amino acids, emphasizing the importance of molecular weight and isotopic abundance.
Explores beam-matter interactions, focusing on emission phenomena from core electron ionization by X-Rays and electrons, and the competition between Auger and X-Rays emissions.
Explains chromatography principles for protein purification, covering peak broadening causes, band broadening components, and common stationary phases.