Introduces fundamental concepts of fluorescence in microscopy, covering terms like excitation and emission spectra, Stokes shift, quantum efficiency, brightness, and photobleaching.
Analyzes a mutant DDX3 enzyme's ATPase activity and inhibitor development, covering RNA chaperones, crystal structures, and mass spectrometry-based protein profiling.
Covers the introduction to fluorescence in microscopy, labeling techniques, specificity of markers, multi-color imaging, and factors affecting fluorescence properties.